Quality control for antibodies

Antibodies are among the most frequently used tools in the biosciences, as they enable researchers to identify molecules. However, many commercial antibodies are not specific enough, with the result that they do not recognize the molecules they are supposed to target. A team led by Dr. Johan Duchêne (Institute for Cardiovascular Prevention (IPEK), member of the LMU Open Science Center) has now investigated commercial antibodies against the molecule ACKR1, which is expressed on red blood cells, endothelial cells, and some neurons. On endothelial cells, it is an important regulator of inflammation, as it controls the migration of white blood cells into tissue. It had been shown that the malaria pathogen uses ACKR1 to infect red blood cells. A study from 2016 reported furthermore that ACKR1 is also expressed on monocytes and macrophages. This would have influenced prevailing assumptions about the pathophysiological functions of ACKR1 and could raise the question, for example, as to whether the malaria parasite also infects macrophages and monocytes.

Duchêne has now refuted this study by demonstrating that the antibodies do not recognize the molecule and ACKR1 is not expressed by macrophages and monocytes. Reproducibility and replicability of research results are important quality criteria in science. As the authors emphasize, their study exemplifies the importance for the scientific community of publishing corrections.

Antal Rot, Julia C. Gutjahr, Aindrila Biswas, Maria Aslani, Elin Hub, Aude Thiriot, Ulrich H. von Andrian, Remco T.A. Megens, Christian Weber, and Johan Duchêne: Murine bone marrow macrophages and human monocytes do not express atypical chemokine receptor. Cell Stem Cell 2022